Get Started

With In Situ Sequencing Kit

Check that you have the optimal imaging settings for ISS

Check that all required material and reagents are available

Download the ISS Kit Checklist

Image the HS Library Preparation and DAPI counterstaining

Use a standard fluorescent microscope with a 20X objective
to image the DAPI counterstaining and all the ISS spots.

It is recommended to work in an RNase free environment to avoid degradation of RNA molecules. Always use fresh RNAse free reagents for  samples preparation.