In Situ Sequencing (ISS) is a new and innovative methods of nucleic acid analysis performed directly in situ on the tissue section, preserving the spatial information.
The CARTANA ISS technology uses gene-specific barcoded padlock probes to target hundreds of gene expressed in situ. Once hybridized to the targeted RNA, the padlock probes are ligated and amplified in situ through rolling circle amplification.
Next generation sequencing chemistry is then applied in situ, directly on the tissue section, to sequence and decode the padlock probes barcode and identify the targeted RNA. Our analysis software package decodes the barcode sequences, thereby identifying and quantifying the genes.
The result is a map of gene expression data of all analysed genes and their abundance with sub-cellular resolution within the morphological context.
Using our ISS technology researchers can easily target, localize and validate more up to 600 genes with just one tissue sample in one experiment. Our technology ensures high performance (high specificity and high throughput) and reliable reproducible data.
In Situ Sequencing Technology
“Single cell transcriptomics has enabled molecular understanding of the fundamental building block of life now its time to take the next step and understand how these building blocks act together in tissues and organs, which is the functional unit of animals. Let’s go from molecular cell biology to molecular tissue biology!”
-Professor Mats Nilsson, P.h.D, Science For Life Laboratory, Stockholm University